Untargeted metabolomics analysis reveals the metabolic disturbances and exacerbation of oxidative stress in recurrent spontaneous abortion

• DOI: 10.1371/journal.pone.0296122
• Publication Date: 2023-12-21T18:49:38Z
• Authors: AiNing Wu; YanHui Zhao; RongXin Yu; JianXing Zhou; Ya Tuo
• Keywords: Metabolome; Metabolic pathway

Recurrent spontaneous abortion (RSA) is characterized by the occurrence of two or more consecutive abortions, with a rising prevalence among pregnant women and significant implications for their physical mental well-being. The multifaceted etiology RSA has posed challenges in unraveling molecular mechanisms underlying that underlie its pathogenesis. Oxidative stress immune response have been identified as pivotal factors development condition.Eleven serum samples from healthy 17 were subjected to liquid chromatography/mass spectrometry (LC-MS) analysis. Multivariate statistical analysis was employed excavate system-level characterization metabolome. measurement seven oxidative products, namely superoxide dismutase (SOD), catalase (CAT), malonaldehyde (MDA), glutathione (GPx), peroxidase (GSH), oxidized (GSSG), heme oxygenase (HO-1), carried out using ELISA.Through monitoring metabolic lipid alternations during events, we 816 biomarkers implicated various pathways, including metabolism, phosphonate phosphinate nucleotide sphingolipid lysine degradation purine etc. These pathways found be closely associated progression disease. Our finding indicated levels MDA HO-1 elevated group compared control group, whereas SOD, CAT GPx exhibited contrary pattern. However, no slight difference observed GSH GSSG between group.The manifestation elicited discernible temporal metabolome biochemical markers linked response. investigation furnished comprehensive analytical framework encompassing metabolites enzymes stress. This inquiry nuanced comprehension pathogenesis established ground work prognostication prophylaxis.