Isolation of plant-derived exosome-like nanoparticles (PDENs) from Solanum nigrum L. berries and Their Effect on interleukin-6 expression as a potential anti-inflammatory agent

• DOI: 10.1371/journal.pone.0296259
• Publication Date: 2024-01-04T18:24:14Z
• Authors: Natasya Emmanuela; Daisy Ramadhani M...; Iriawati; Christofora Hanny...; Yuliana Maria Dia...; Hiroshi Takemori; Ika Dewi Ana; Ratna Yuniati; Windri Handayani; Triati Dewi Kenca...; Yasuhiko Tabata; Anggraini Barlian
• Keywords: Exosome; Solanum nigrum; Extracellular vesicles

Inflammation is a temporary response of the immune system that can be treated using common anti-inflammatory drugs. However, prolonged use these drugs increases risk adverse side effects. Accordingly, there an increasing need for alternative treatments inflammation with fewer Exosomes are extracellular vesicles secreted by most eukaryotic cells and have been studied as candidate cell-free therapy inflammatory diseases due to their immunomodulatory properties. In recent years, focus exosome research has shifted from animal cell-derived exosomes plant-derived exosome-like nanoparticles (PDENs). Plant-derived (PDENs) easier obtain, minimal safety concerns, produced in higher quantities lower cost than derived cells. this study, isolation analysis potential PDENs black nightshade berries ( Solanum nigrum L.) were carried out. The results characterization showed had spherical morphology, measuring around 107 nm zeta -0.6 mV, protein concentration 275.38 μg/mL. also shown internalized RAW264.7 macrophage cell line after 2 hours incubation no cytotoxicity effect up 2.5 Furthermore, exposure several doses LPS-stimulated significantly decreased expression pro-inflammatory cytokine gene IL-6, well IL-6 97,28%. GC-MS presence neral, monoterpene compound known properties, which may contribute activity isolated L. berries. Taken together, present study was first isolate characterize demonstrated PDEN suppressing production