Method to obtain a plasma rich in platelet- and plasma-growth factors based on water evaporation

• DOI: 10.1371/journal.pone.0297001
• Publication Date: 2024-02-21T18:21:09Z
• Authors: Jon Mercader Ruiz; Maider Beitia; Diego Delgado; Pello Sánchez; Miren Begoña Sánchez; Jaime Oraa; Fernando Benito-L...; Lourdes Basabe-De...; Mikel Sánchez
• Keywords: Blood Platelets; Wound Healing; Platelet-Rich Plasma; Science; Q; R; Medicine; Intercellular Signaling Peptides and Proteins; Research Article

Platelet-Rich Plasma, also known as PRP, is an autologous biologic product used in medicine as a treatment for tissue repair. Nowadays, the majority of PRP obtention methods enrich only platelets, not considering extraplatelet biomolecules, which take part in several cell processes. In the present work, a novel PRP preparation method was developed to obtain a PRP rich in both platelet and plasma extraplatelet molecules. The method is based on the evaporation of the water of the plasma using a rotary evaporator. With this new methodology an increase in plasmatic growth factors and, as a consequence, a better dermal fibroblast cell viability was achieved, compared to a standard PRP formulation. This novel PRP product obtained with this new methodology showed promising results in vitro as an improved PRP treatment in future application.