The effects of L-carnitine and fructose in improved Ham’s F10 on sperm culture in idiopathic severe asthenospermia within 24h

• DOI: 10.1371/journal.pone.0306235
• Publication Date: 2025-02-10T18:31:32Z
• Authors: Dehui Chang; Fudong Li; Yindong Kang; Yixin Yan; Feiyan Kong; Wei Jiang; Dongxing Wang; Zhigang Cao; Liuting Xu; Chuang Huang; Yafen Kang; Xuejun Shang; Bin Zhang

To study the effects of L-carnitine and fructose on semen parameters severe asthenospermia patients by sperm culturing in vitro within 24h. We optimized energy composition antioxidant substances culture medium (based Ham’s F10 medium) orthogonal test for preparing high quality medium. Sperms 60 with idiopathic were collected, cultured 24h, added to different concentrations temperature, whose motility observed determine which is most appropriate concentration temperature. For determining suitable temperature medium, experiments carried out optimize above three factors, had great influence viability, survival rate, deformity rate DNA fragmentation index (DFI). The final was determined terms initial tests assess (4, 8, 12, 16 mg/ml 0.125, 0.250, 0.375, 0.50 fructose) viability culture. During operation processing sperms showed that better at 8 0.375 improved 36.5°C. Idiopathic can be effectively modified 36.5°C has shown effect superior basic