Peptidoglycan recognition protein PGRP-5 is involved in immune defence and neuro-behavioral disorders in zebrafish embryos

• DOI: 10.1371/journal.pone.0315714
• Publication Date: 2025-01-31T18:38:17Z
• Authors: Xi Li; Guanghua Xiong; Manni Luo; Siwan Mao; Ruiying Zhang; Ziwei Meng; Juan Li; Xinjun Liao
• Keywords: Science; Q; R; Medicine; Research Article

Peptidoglycan recognition proteins (PGRPs) are the evolutionarily highly conserved class of pattern receptors, however, their functions on innate immune system and neuro-inflammatory response in aquatic organism still poorly understood. In this study, we systematically investigated molecular PGRPs zebrafish embryos. Firstly, identified three phylogenetic analysis suggested that DrPGRP-5 was a novel member PGRP superfamily evolution. Secondly, endogenous mRNA levels were expressed brain muscle while significantly down-regulated liver egg at 72 hpf Thirdly, greatly elevated after 6 h E. coli infection but reached its highest value 24 M. luteus stimulation. Moreover, knock-down could reduce pro-inflammatory cytokines such as TNF-α, IL-1β IL-6, increased expression anti-inflammatory cytokine TGF-β. On other hand, locomotor behavior abilities antioxidant enzyme activities CAT SOD obviously decreased under KD conditions. Finally, incubation embryos with neuroprotective agents (10 μM Minocycline) can partially rescue DrPGRP5-regulated behavior. Taken together, our data PGRP-5 is involved defenses regulated neurobehavior neuro-inflammation, which may provide new strategies for treatment diseases organisms.