Transcriptomic profile induced by calcitriol in CaSki human cervical cancer cell line

• DOI: 10.1371/journal.pone.0319812
• Publication Date: 2025-04-01T20:56:14Z
• Authors: Euclides Avila; Luis David Hernán...; Alberto Cedro-Tanda; Tomas Misael Liza...; David Barrera; Francisco Vladimi...; Janice García-Quiroz; Lorenza Díaz; Fernando Larrea

The vitamin D endocrine system, primarily mediated by its main metabolite calcitriol and the receptor (VDR), plays a critical role in numerous human physiological processes, ranging from calcium metabolism to prevention of various tumors, including cervical cancer. In this study, we comprehensively investigated genomic regulatory effects cancer model. We examined transcriptional changes induced CaSki cells, cell line harboring multiple copies HPV16, primary causal agent Our microarray findings, revealed that regulated over 1000 protein-coding genes, exhibiting predominantly repressive effect on transcriptome suppressing twice as many genes it induced. Calcitriol decreased EPHA2 RARA expression while inducing KLK6 CYP4F3 validated qPCR Western blot. Functional analysis demonstrated effectively inhibited key processes involved progression, proliferation migration. This was further supported significant downregulation MMP7 MMP13 mRNA levels. results also showed that, addition significantly regulates non-coding RNAs, altering approximately 400 111 microRNA precursors 29 mature microRNAs, which 17 were upregulated 12 downregulated. Notably, among these calcitriol-regulated microRNAs are some biology, such miR-6129, miR-382, miR-655, miR-211, miR-590, miR-130a, miR-301a, miR-1252. Collectively, findings suggest exhibits antitumor advanced model blocking for tumor underscoring importance maintaining adequate nutritional status.