Combining ChIP-chip and Expression Profiling to Model the MoCRZ1 Mediated Circuit for Ca2+/Calcineurin Signaling in the Rice Blast Fungus

• DOI: 10.1371/journal.ppat.1000909
• Publication Date: 2010-05-20T19:05:42Z
• Authors: Soonok Kim; Jinnan Hu; Yeonyee Oh; Jongsun Park; Jinhee Choi; Yong-Hwan Lee; Ralph A. Dean; Thomas K. Mitchell
• Keywords: Calcium Signaling; Chromatin immunoprecipitation

Significant progress has been made in defining the central signaling networks many organisms, but collectively we know little about downstream targets of these and genes they regulate. To reconstruct regulatory circuit calcineurin signal transduction via MoCRZ1, a Magnaporthe oryzae C2H2 transcription factor activated by dephosphorylation, used combined approach chromatin immunoprecipitation - chip (ChIP-chip), coupled with microarray expression studies. One hundred forty were identified as being both direct target MoCRZ1 having concurrently differentially regulated calcium/calcineurin/MoCRZ1 dependent manner. Highly represented involved calcium signaling, small molecule transport, ion homeostasis, cell wall synthesis/maintenance, fungal virulence. Of particular note, vesicle mediated secretion necessary for establishing host associations, also found. itself was target, suggesting previously unreported autoregulation control point. The data implicated feedback regulation mechanism activity. We propose that calcium/calcineurin circuits controlling development pathogenicity manifest through multiple layers regulation. present results from ChIP-chip analysis along refined model this important plant pathogen.