Probiotic Bifidobacterium breve Induces IL-10-Producing Tr1 Cells in the Colon

• DOI: 10.1371/journal.ppat.1002714
• Publication Date: 2012-05-31T21:05:05Z
• Authors: Seong Gyu Jeon; Hisako Kayama; Yoshiyasu Ueda; Takuya Takahashi; Takashi Asahara; Hirokazu Tsuji; Noriko M. Tsuji; Hiroshi Kiyono; Ji Su Ma; Takashi Kusu; Ryu Okumura; Hiromitsu Hara; Hiroki Yoshida; Masahiro Yamamoto; Koji Nomoto; Kiyoshi Takeda
• Keywords: Bifidobacterium breve; Lamina propria; Regulatory B cells; Lactobacillus casei

Specific intestinal microbiota has been shown to induce Foxp3+ regulatory T cell development. However, it remains unclear how development of another subset, Tr1 cells, is regulated in the intestine. Here, we analyzed role two probiotic strains bacteria, Lactobacillus casei and Bifidobacterium breve B. breve, but not L. casei, induced IL-10-producing cells that express cMaf, IL-21, Ahr large Intestinal CD103+ dendritic (DCs) mediated breve-induced cells. DCs from Il10−/−, Tlr2−/−, Myd88−/− mice showed defective breve-treated failed IL-10 production co-cultured Il27ra−/− treatment Tlr2−/− did increase colonic lamina propria. Thus, activates produce IL-27 via TLR2/MyD88 pathway thereby inducing Oral administration ameliorated colitis immunocompromised given naïve CD4+ wild-type mice, Il10−/− mice. These findings demonstrate prevents inflammation through induction