A Compositional Look at the Human Gastrointestinal Microbiome and Immune Activation Parameters in HIV Infected Subjects

• DOI: 10.1371/journal.ppat.1003829
• Publication Date: 2014-02-20T22:15:52Z
• Authors: Ece A. Mutlu; Ali Keshavarzian; John Losurdo; Garth Swanson; Basile Siewe; Christopher Forsyth; Audrey French; Patricia DeMarais; Yan Sun; Lars Koenig; Stephen Cox; Phillip Engen; Prachi Chakradeo; Rawan Abbasi; Annika Gorenz; Charles Burns; Alan Landay
• Keywords: Dysbiosis; Pyrosequencing

HIV progression is characterized by immune activation and microbial translocation. One factor that may be contributing to could a dysbiotic microbiome. We therefore hypothesized the GI mucosal microbiome altered in patients this alteration correlates with HIV. 121 specimens were collected from 21 positive 22 control human subjects during colonoscopy. The composition of lower gastrointestinal tract luminal bacterial was using 16S rDNA pyrosequencing correlated clinical parameters as well circulating products on ART. significantly different than controls; it less diverse right colon terminal ileum, loss taxa are typically considered commensals. In samples, there gain some pathogenic taxa. This first report characterizing ileal colonic next generation sequencing. Limitations include use HIV-infected HAART therapy.