Co-opted Oxysterol-Binding ORP and VAP Proteins Channel Sterols to RNA Virus Replication Sites via Membrane Contact Sites

• DOI: 10.1371/journal.ppat.1004388
• Publication Date: 2014-10-20T21:28:27Z
• Authors: Daniel Barajas; Kai Xu; Isabel Fernández ...; Zsuzsanna Sasvari; Federica Brandizzi; Cristina Risco; Peter D. Nagy
• Keywords: Replication factor C

Viruses recruit cellular membranes and subvert proteins involved in lipid biosynthesis to build viral replicase complexes replication organelles. Among the lipids, sterols are important components of membranes, affecting shape curvature membranes. In this paper, tombusvirus protein is shown co-opt Oxysterol-binding related (ORPs), whose deletion yeast model host leads decreased replication. addition, tombusviruses also Scs2p VAP facilitate formation membrane contact sites (MCSs), where juxtaposed, likely channeling lipids sites. all, these events result redistribution enrichment at plant cells. Using vitro assay with artificial vesicles, we show stimulation by sterols. Thus, co-opting ORP form MCSs serves virus need generate abundant sterol-rich surfaces for