Escherichia coli α-Hemolysin Counteracts the Anti-Virulence Innate Immune Response Triggered by the Rho GTPase Activating Toxin CNF1 during Bacteremia

• DOI: 10.1371/journal.ppat.1004732
• Publication Date: 2015-03-17T20:00:58Z
• Authors: Mamady Diabate; Patrick Munro; Elsa Garcia; Arnaud Jacquel; Gregory Michel; Sandrine Obba; Diogo Goncalves; Carmelo Luci; Sandrine Marchetti; Dieter Demon; Clara Degos; Yassina Bechah; Jean-Louis Mege; Mohamed Lamkanfi; Patrick Auberger; Jean-Pierre Gorvel; Lynda Maria Stuart; Luce Landraud; Emmanuel Lemichez; Laurent Boyer
• Keywords: Hemolysin

The detection of the activities pathogen-encoded virulence factors by innate immune system has emerged as a new paradigm pathogen recognition. Much remains to be determined with regard molecular and cellular components contributing this defense mechanism in mammals importance during infection. Here, we reveal central role IL-1β signaling axis Gr1+ cells controlling Escherichia coli burden blood response sensing Rho GTPase-activating toxin CNF1. Consistently, is abrogated caspase-1/11-impaired mice or following treatment infected an antagonist. In vitro experiments further revealed synergistic effects CNF1 LPS promoting maturation/secretion establishing roles Rac, ASC caspase-1 pathway. Furthermore, found that α-hemolysin inhibits secretion without affecting recruitment cells. report first example anti-virulence-triggered immunity counteracted pore-forming bacteremia.