The small non-coding RNA RsaE influences extracellular matrix composition in Staphylococcus epidermidis biofilm communities

• DOI: 10.1371/journal.ppat.1007618
• Publication Date: 2019-03-14T14:41:51Z
• Authors: Sonja M. K. Schoe...; Claudia Lange; Srinivasa Abishek...; Gabriella Marincola; Maike F. Lerch; Freya D. R. Wencker; Konrad U. Förstner; Cynthia M. Sharma; Wilma Ziebuhr
• Keywords: 0301 basic medicine; ddc:610; QH301-705.5; Gene Expression Profiling; Staphylococcus; Polysaccharides, Bacterial; Gene Expression Regulation, Bacterial; RC581-607; Staphylococcal Infections; Extracellular Matrix; 3. Good health; 03 medical and health sciences; Phenotype; Bacterial Proteins; Biofilms; Operon; Staphylococcus epidermidis; RNA, Small Untranslated; Immunologic diseases. Allergy; Biology (General); Research Article

RsaE is a conserved small regulatory RNA (sRNA) which was previously reported to represent riboregulator of central carbon flow and other metabolic pathways in Staphylococcus aureus Bacillus subtilis. Here we show that contributes extracellular (e)DNA release biofilm-matrix switching towards polysaccharide intercellular adhesin (PIA) production hypervariable epidermidis isolate. Transcriptome analysis through differential sequencing (dRNA-seq) combination with confocal laser scanning microscopy (CLSM) reporter gene fusions demonstrate S. protein- PIA-biofilm matrix producers differ respect expression. spatiotemporally expressed within PIA-mediated biofilms, its overexpression triggers PIA biofilm phenotype as well eDNA an protein matrix-producing strain background. dRNA-seq Northern blot analyses revealed exist major full-length 100-nt transcript minor processed species lacking approximately 20 nucleotides at the 5'-end. processing results expansion mRNA target spectrum. Thus, interacts antiholin-encoding lrgA mRNA, facilitating bacterial lysis release. Processed RsaE, however, 5'-UTR icaR sucCD mRNAs, encoding icaADBC operon repressor IcaR succinyl-CoA synthetase tricarboxylic acid (TCA) cycle, respectively. augments production, most likely activation expression via repression by TCA cycle inhibition re-programming staphylococcal metabolism precursor synthesis. Additionally, supports formation mediating stabilizing component. As itself heterogeneously consider this sRNA function factor favoring phenotypic heterogeneity supporting division labor communities.