A shear stress micromodel of urinary tract infection by the Escherichia coli producing Dr adhesin

• DOI: 10.1371/journal.ppat.1008247
• Publication Date: 2020-01-09T13:36:51Z
• Authors: Beata Zalewska-Pi...; Marcin Olszewski; Tomasz Lipniacki; Sławomir Błoński; Miłosz Wieczór; Piotr Bruździak; Anna Skwarska; Bogdan Nowicki; Stella Nowicki; Rafał Piątek
• Keywords: Micromodel

In this study, we established a dynamic micromodel of urinary tract infection to analyze the impact UT-segment-specific outflow on persistence E. coli colonization. We found that adherence Dr+ bladder T24 transitional cells and type IV collagen is maximal at lowest shear stress reduced by any increase in flow velocity. The analyzed was effective whole spectrum physiological almost irreversible over entire range generated force. Once bound host or collagen, they did not detach even presence elevated chloramphenicol, competitive inhibitor binding. Investigating role epithelial surface architecture, showed budding cells-a model microarchitectural obstacle-promotes colonization coli. report previously undescribed phenomenon cell "rolling-shedding" colonization, which detached reattach underlying line through layer adherent This rolling-shedding progressed continuously due "refilling" induced flow-perturbing obstacle. fluid containing free-floating bacteria fueled rolling, while providing an uninterrupted supply new be trapped rolling cell. progressive allows for transfer briefly attached onto monolayer repeating cascading event.