Quantitative proteomics analysis reveals important roles of N-glycosylation on ER quality control system for development and pathogenesis in Magnaporthe oryzae

• DOI: 10.1371/journal.ppat.1008355
• Publication Date: 2020-02-24T18:49:03Z
• Authors: Xiao-Lin Chen; Caiyun Liu; Bozeng Tang; Zhiyong Ren; Guo-Liang Wang; Wende Liu
• Keywords: Conidiation; Appressorium; Magnaporthe; N-linked glycosylation; Fungal protein

Genetic studies have shown essential functions of N-glycosylation during infection the plant pathogenic fungi, however, systematic roles in fungi is still largely unknown. Biological analysis demonstrated N-glycosylated proteins were widely present at different development stages Magnaporthe oryzae and especially increased appressorium invasive hyphae. A large-scale quantitative proteomics was then performed to explore M. oryzae. total 559 N-glycosites from 355 identified quantified developmental stages. Functional classification revealed can coordinate cellular processes for mycelial growth, conidium formation, formation. also modify key components N-glycosylation, O-glycosylation GPI anchor pathways, indicating intimate crosstalk between these pathways. Interestingly, we found nearly all endoplasmic reticulum quality control (ERQC) system highly appressorium. Phenotypic analyses gene deletion mutants four ERQC components, Gls1, Gls2, GTB1 Cnx1, are important conidiation, hyphal growth host cells. Subsequently, Gls1 N-glycosite N497 partially required but didn't affect colony growth. Mutation resulted reduction protein level, localization ER into vacuole, suggesting stability Gls1. Our study showed a snapshot landscape this modification processes, developments pathogenesis.