Characterizing Emerging Canine H3 Influenza Viruses

• DOI: 10.1371/journal.ppat.1008409
• Publication Date: 2020-04-14T17:35:32Z
• Authors: Luis Martinez-Sob...; Pilar Blanco-Lobo; Laura Rodriguez; Theresa Fitzgerald; Hanyuan Zhang; Phuong Nguyen; Christopher S. An...; Jeanne Holden-Wiltse; Sanjukta Bandyopa...; Aitor Nogales; Marta L. DeDiego; Brian R. Wasik; Benjamin L. Miller; Carole Henry; Patrick C. Wilson; Mark Y. Sangster; John J. Treanor; David J. Topham; Lauren Byrd-Leotis; David A. Steinhauer; Richard D. Cummings; Jasmina M. Luczo; Stephen M. Tompkins; Kaori Sakamoto; Cheryl A. Jones; John Steel; Anice C. Lowen; Shamika Danzy; Hui Tao; Ashley L. Fink; Sabra L. Klein; Nicholas Wohlgemuth; Katherine J. Fens...; Farah el Najjar; Andrew Pekosz; Lauren Sauer; Mitra K. Lewis; Kathryn Shaw-Saliba; Richard E. Rothman; Zhen-Ying Liu; Kuan-Fu Chen; Colin R. Parrish; Ian E. H. Voorhees; Yoshihiro Kawaoka; Gabriele Neumann; Shiho Chiba; Shufang Fan; Masato Hatta; Huihui Kong; Gongxun Zhong; Guojun Wang; Melissa B. Uccellini; Adolfo García-Sastre; Daniel R. Perez; Lucas M. Ferreri; Sander Herfst; Mathilde Richard; Ron Fouchier; David Burke; David Pattinson; Derek J. Smith; Victoria Meliopoulos; Pamela Freiden; Brandi Livingston; Bridgett Sharp; Sean Cherry; Juan Carlos Dib; Guohua Yang; Charles J. Russell; Subrata Barman; Richard J. Webby; Scott Krauss; Angela Danner; Karlie Woodard; Malik Peiris; R. A. P. M. Perera; M. C. W. Chan; Elena A. Govorkova; Bindumadhav M. Ma...; Philippe N. Q. Pa...; Gavin Smith; Yao-Tsun Li; Paul G. Thomas; Stacey Schultz-Ch...
• Keywords: 0301 basic medicine; QH301-705.5; EMC OR-01; Guinea Pigs; Communicable Diseases, Emerging; Influenza A Virus, H3N8 Subtype; 03 medical and health sciences; Dogs; Zoonoses; Influenza, Human; Animals; Humans; Dog Diseases; Biology (General); Medicine and health sciences; Mice, Inbred BALB C; 0303 health sciences; Biology and life sciences; Influenza A Virus, H3N2 Subtype; Ferrets; RC581-607; United States; 3. Good health; Mice, Inbred C57BL; Research and analysis methods; Influenza A virus; Mice, Inbred DBA; Immunologic diseases. Allergy; Research Article

The continual emergence of novel influenza A strains from non-human hosts requires constant vigilance and the need for ongoing research to identify strains that may pose a human public health risk. Since 1999, canine H3 influenza A viruses (CIVs) have caused many thousands or millions of respiratory infections in dogs in the United States. While no human infections with CIVs have been reported to date, these viruses could pose a zoonotic risk. In these studies, the National Institutes of Allergy and Infectious Diseases (NIAID) Centers of Excellence for Influenza Research and Surveillance (CEIRS) network collaboratively demonstrated that CIVs replicated in some primary human cells and transmitted effectively in mammalian models. While people born after 1970 had little or no pre-existing humoral immunity against CIVs, the viruses were sensitive to existing antivirals and we identified a panel of H3 cross-reactive human monoclonal antibodies (hmAbs) that could have prophylactic and/or therapeutic value. Our data predict these CIVs posed a low risk to humans. Importantly, we showed that the CEIRS network could work together to provide basic research information important for characterizing emerging influenza viruses, although there were valuable lessons learned.