Acute experimental infection of bats and ferrets with Hendra virus: Insights into the early host response of the reservoir host and susceptible model species

• DOI: 10.1371/journal.ppat.1008412
• Publication Date: 2020-03-30T17:27:56Z
• Authors: Amanda P. Woon; Victoria Boyd; Shawn Todd; Ina Smith; Reuben Klein; Isaac B. Woodhouse; Sarah Riddell; Gary Crameri; John Bingham; Lin-Fa Wang; Anthony W. Purcell; Deborah Middleton; Michelle L. Baker
• Keywords: Hendra Virus; Viral Shedding

Bats are the natural reservoir host for a number of zoonotic viruses, including Hendra virus (HeV) which causes severe clinical disease in humans and other susceptible hosts. Our understanding ability bats to avoid following infection with viruses such as HeV has come predominantly from vitro studies focusing on innate immunity. Information early response vivo is lacking there no comparative data responses compared animals that succumb disease. In this study, we examined sites replication immune infected Australian black flying foxes ferrets at 12, 36 60 hours post exposure (hpe). Viral antigen was detected hpe confined lungs whereas evidence widespread viral RNA by hpe. The mRNA expression IFNs revealed antagonism type I III significant increase chemokine, CXCL10, bat lung spleen infection. ferrets, an transcription IFN Liquid chromatography tandem mass spectrometry (LC-MS/MS) tissue performed 0 obtain global overview protein expression. Gene Ontology (GO) enrichment analysis pathways six pathways, involved cell mediated immunity were more likely be upregulated ferrets. GO also signaling pathway This study contributes important differences dissemination first provide activation