Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage

• DOI: 10.1371/journal.ppat.1008840
• Publication Date: 2020-09-10T17:25:28Z
• Authors: Natália Satchiko ...; Patrick Orestes d...; Júlia Teixeira de...; Andréa Teixeira-C...; Judy Lieberman; Caroline Junqueira; Ricardo Tostes Ga...
• Keywords: Plasmodium yoelii; Granzyme

P. vivax-infected Retics (iRetics) express human leukocyte antigen class I (HLA-I), are recognized by CD8+ T cells and killed granulysin (GNLY) granzymes. However, how Plasmodium infection induces MHC-I expression on is unknown. In addition, whether GNLY helps control in vivo has not been studied. Here, we examine these questions using rodent with the yoelii 17XNL strain, which tropism for Retics. Infection caused extramedullary erythropoiesis, reticulocytosis expansion of CD8+CD44+CD62L- IFN-γ-producing that form immune synapses iRetics. We now provide evidence iRetic dependent IFN-γ-induced transcription IRF-1, β2-microglobulin (β2-m) erythroblasts. Consistently, CTLs from infected wild type (WT) mice formed iRetics an IFN-γ- MHC-I-dependent manner. When challenged 17XNL, WT cleared parasitemia survived, while IFN-γ KO remained parasitemic all died. β2-m do have virtually no had prolonged parasitemia, 80% survived. Because GNLY, GNLY-transgenic can be used to assess importance GNLY. Parasite clearance was accelerated depletion ablated GNLY-mediated resistance yoelii. Altogether, our results indicate addition previously described mechanisms, promotes host Retic-tropic strain promoting become targets cytotoxic lymphocytes