Respiratory syncytial virus ribonucleoproteins hijack microtubule Rab11 dependent transport for intracellular trafficking

570 0303 health sciences QH301-705.5 [SDV]Life Sciences [q-bio] Endosomes RC581-607 Microtubules 3. Good health [SDV] Life Sciences [q-bio] Protein Transport Viral Proteins 03 medical and health sciences Ribonucleoproteins rab GTP-Binding Proteins Respiratory Syncytial Virus, Human Humans Immunologic diseases. Allergy Biology (General) Research Article
DOI: 10.1371/journal.ppat.1010619 Publication Date: 2022-07-07T17:41:07Z
ABSTRACT
Respiratory syncytial virus (RSV) is the primary cause of severe respiratory infection in infants worldwide. Replication RSV genomic RNA occurs cytoplasmic inclusions generating viral ribonucleoprotein complexes (vRNPs). vRNPs then reach assembly and budding sites at plasma membrane. However, mechanisms ensuring transportation are unknown. We generated a recombinant harboring fluorescent RNPs allowing us to visualize moving living infected cells developed an automated imaging pipeline characterize movements high throughput. Automatic tracking revealed that around 10% exhibit fast directed motion compatible with transport along microtubules. Visualization labeled microtubules restriction their by microtubule depolymerization further support involvement trafficking. Approximately 30% colocalize Rab11a protein, marker endosome recycling (ER) pathway we observed Rab11-labeled vesicles together. Transient inhibition expression significantly reduces demonstrating Rab11 Finally, specifically immunoprecipitated suggesting interaction between vRNPs. Altogether, our results strongly suggest move on hijacking ER pathway.
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