Inter-Laboratory Robustness of Next-Generation Bile Acid Study in Mice and Humans: International Ring Trial Involving 12 Laboratories

• DOI: 10.1373/jalm.2016.020537
• Publication Date: 2018-07-23T22:25:47Z
• Authors: Hai T Pham; Kathrin Arnhard; Yasmin J Asad; Lu Deng; Thomas K Felder; Lisa St John-Will...; Volkhard Kaever; Michael Leadley; Nico Mitro; Stephane Muccio; Cornelia Prehn; Manfred Rauh; Ulrike Rolle-Kamp...; J Will Thompson; Olaf Uhl; Maria Ulaszewska; Michael Vogeser; David S Wishart; Therese Koal
• Keywords: Robustness

Abstract Background The increasing relevance of individual bile acids quantification in biological samples requires analytical standardization to guarantee robustness and reliability laboratory results. We have organized the first international ring trial, carried out 12 laboratories, evaluate newly developed LC-MS/MS–based test kit for acid analysis. Methods Each received a Biocrates® Bile Acids Kit including system suitability (SST) protocol. is designed analyze 16 human 19 mouse acids. A set 9 plasma was measured replicates. Laboratories were required pass acceptance criteria SST. Within subset laboratories passing SST criteria, we evaluated how many met target 80% reported values with relative accuracy within 70%–130% range precisions (%CV) below 30%. Results total participating passed as prerequisite enter trial. All then able successfully run trial samples. Of overall values, 94% range. Mean precision 8.3% CV. condition CV <30% fulfilled by 99% values. Conclusions publically available interlaboratory standardized showed very good performance, typically applied preclinical environment. therefore suitable quantitative analysis establishment reference