Animal Models for Disease: Knockout, Knock-In, and Conditional Mutant Mice

• DOI: 10.1385/1-59745-213-0:41
• Publication Date: 2006-11-04T17:01:44Z
• Authors: David F. LePage; Ronald A. Conlon
• Keywords: Gene knockout; Conditional gene knockout; Gene knockin; Knockout mouse; Gene targeting

Diseases with a genetic basis can be modeled knockout, knock-in, and conditional mutant gene-targeted mice. In the following, we provide detailed protocols for gene targeting. Gene targeting of embryonic stem cells accomplished by laboratories equipped tissue culture. Alternatively, many gene-targeting services divide work customer lab. this collaborative situation, knowledge entire process helps ensure successful outcome. The construction chimeras germ-line transmission is not described here, because procedure beyond means most laboratories, typically provided transgenic core facilities, best learned through hands-on demonstration.