Comparison of ITS-1 and TBR-1/2 primer sensitivity for the detection of Trypanosoma evansi local isolates in experimental rats using a polymerase chain reaction

• DOI: 10.14202/vetworld.2022.1772-1778
• Publication Date: 2022-07-24T07:30:49Z
• Authors: Endang Suprihati; Lucia Tri Suwanti; Aditya Yudhana; Andika Indra Kusu...
• Keywords: Trypanosoma evansi; Primer (cosmetics)

Background and Aim: Surra is caused by Trypanosoma evansi. The detection method using conventional parasitological tests has not always shown positive results in blood parasite detection, although the livestock presented with clinical signs. Therefore, a fast accurate diagnosis necessary to prevent disease predominately field isolates. This study aimed investigate sensitivity of molecular two different specific primers, namely, Internal Transcribed Spacer 1 (ITS-1) brucei repeat 1/2 (TBR-1/2) against T. evansi isolates from Banten Province, Indonesia. Materials Methods: used this were collected Province cultured preserved National Research Center for Veterinary Science, Eighteen experimental rats divided into three equal groups, which categorized as control, × 101, 104 infective doses. injected all albino intraperitoneally. All samples tested smear, card agglutination test (CATT), polymerase chain reaction (PCR) method. Results: CATT examination treatments showed negative results. However, PCR that ITS-1 TBR-1/2 had been successfully detected infected rats, proven band appeared 480 base pairs (bp) 164 bp, respectively. Conclusion: Based on diagnostic method, primer more sensitive detect compared primer. present finding provides preliminary data studying efficiency primers if practically applied standard trypanosomiasis, especially Indonesian livestock.