A rabbit model of ear otitis established using the Malassezia pachydermatis strain C23 from dogs

• DOI: 10.14202/vetworld.2023.2192-2199
• Publication Date: 2023-11-04T06:34:00Z
• Authors: Nadezhda Sachivkina; Arfenya Karamyan; Olesya Petrukhina; Olga Kuznetsova; Ekaterina Neborak; Alfia Ibragimova
• Keywords: Malassezia; Strain (injury); Rabbit (cipher)

Background and Aim: Fungal infections are a growing problem for both humans animals due to the emergence of pathogenic strains resistant modern antifungal treatments. To evaluate efficacy new drugs, it is essential develop animal models that demonstrate typical responses infection (pathogenesis clinical course) treatment, including adverse effects. In this study, we established rabbit otitis model by an aggressive multidrug-resistant strain from dogs, Malassezia pachydermatis C23, with no need concomitant immunosuppression. Materials Methods: Twenty healthy adult male gray giant rabbits (1 year old, 5.5 kg) were inoculated once M. C23 at 108 colony-forming units/mL. We observed signs disease collected ear smears blood samples every 5 days. Results: The progressed rapidly exhibited characteristic without spontaneous recovery least 1 month. fact, substantial deterioration was as evidenced parameters. Conclusion: This using drug-resistant fungus immunosuppression could prove valuable testing novel agents. Keywords: in vivo model, pachydermatis, media, rabbit.