Distribution analysis of tetracycline resistance genes in Escherichia coli isolated from floor surface and effluent of pig slaughterhouses in Banten Province, Indonesia

• DOI: 10.14202/vetworld.2023.509-517
• Publication Date: 2023-03-21T05:59:56Z
• Authors: Debby Fadhilah Pazra; Hadri Latif; Chaerul Basri; I. Wayan Teguh Wi...; Puji Rahayu
• Keywords: 2. Zero hunger; Veterinary medicine; tetracycline resistance; SF600-1100; pig slaughterhouse; escherichia coli; effluent; SF1-1100; 6. Clean water; tet gene; Animal culture; Research Article; 3. Good health

Background and Aim: Slaughterhouses their effluents could serve as a "hotspot" for the occurrence distribution of antibiotic-resistant bacteria in environment. This study aimed to understand tetracycline resistance genes Escherichia coli isolated from floor surface effluent samples pig slaughterhouses Banten Province, Indonesia. Materials Methods: Ten samples, each swabs effluents, were collected 10 Province. strains identified by referring protocol Global Tricycle Surveillance extended-spectrum beta-lactamase E. WHO (2021). Quantitative real-time polymerase chain reaction (qPCR) was used detect tet genes. Results: The tetA, tetB, tetC, tetM, tetO, tetX distributed isolates tetE, samples. tetO gene (60%) most dominant while tetA one (50%). + combination pattern (15%) isolates. Conclusion: high prevalence diversity Province indicated that transmission had occurred pigs environment; thus, this situation should be considered serious threat public health. Keywords: effluent, coli, slaughterhouse, gene, resistance.