First detection of bovine tuberculosis by Ziehl–Neelsen staining and polymerase chain reaction at dairy farms in the Lekok Sub-District, Pasuruan Regency, and Surabaya region, Indonesia

• DOI: 10.14202/vetworld.2024.577-584
• Publication Date: 2024-03-08T11:37:23Z
• Authors: Itfetania Aemilly...; Muhammad Luqman; Yulianna Puspitasari; Wiwiek Tyasningsih; Dhandy Koesoemo W...; Dewa Ketut Meles; Yeni Dhamayanti; Dian Ayu Permatasari; Adiana Mutamsari ...; Agnes Dwi Sis Per...; Hartanto Mulyo Ra...; Siti Rani Ayuti; Shendy Canadya Ku...; Intan Noor Aina K...; Otto Sahat Martua...
• Keywords: Ziehl–Neelsen stain; Bovine tuberculosis; Bovine milk

Bovine tuberculosis (TB) is a zoonotic disease of great public health importance, particularly in Indonesia, where control measures are limited or not implemented. This study aimed to detect the presence Mycobacterium pathogens milk samples from dairy cattle Pasuruan regency and Surabaya City, East Java, using Ziehl-Neelsen acid-fast staining polymerase chain reaction (PCR). Milk were aseptically collected 50 Lekok Subdistrict, Regency, 44 farms Lakarsantri Wonocolo Mulyorejo Kenjeran Surabaya, Java. To Mycobacteria at species level, each sample was assessed by PCR RD1 RD4 genes. The results assay Regency showed that 30 (60%) positive for two (4%) bovis, although did show spp. In region, 31 (70.45%) M. three (6.8%) bovis. Six (13.63%) all PCR-positive could be detected microscopically with Ziehl-Neelsen. bovine TB this supports importance molecular tool alongside routine surveillance better understanding epidemiology