The complete chloroplast genome sequence of Vitis vinifera ‘Krasnostop Zolotovskiy’, an autochthonous variety of the Don Valley

• DOI: 10.14258/turczaninowia.27.1.16
• Publication Date: 2024-06-11T11:02:26Z
• Authors: Fedor S. Sharko; Kristina O. Petrova; Nikolay V. Molchanov; Maxim V. Patrushev; Dmitriy Yu. Fedosov; Stepan V. Toshchakov
• Keywords: Inverted repeat; Sequence assembly

DNA sequence data from complete chloroplast genomes expand our understanding of plant diversity and contributes to evolutionary relationships among species. De novo assembly annotation are difficult due the high number repeats low in international databases. The development long-read sequencing platforms such as PacBio Oxford Nanopore, well specialized assemblers, is simplifying process assembling annotating large circular molecules. In this work, we report genome autochthonous Vitis vinifera variety ‘Krasnostop Zolotovskiy’, obtained de by hybrid method using short long reads. 160,927 bp with a GC ratio 37.38 %. It has four subregions: single-copy (LSC) region 71,946 bp, small (SSC) 36,273 two inverted repeat regions (IRs) 26,354 each. V. Zolotovskiy’ harbours 129 genes, comprising 84 protein-coding 8 rRNA 37 tRNA genes. We also carry out phylogenetic analysis based on all sequences grape available databases, which shows that does not have direct origin any European variety.