Cytohesins in the heart: their expression and interaction with the natriuretic peptide system

• DOI: 10.14293/cgmp.24000034.v1
• Publication Date: 2024-06-05T16:41:08Z
• Authors: Daiana Sedneva-Lu...; Monica Aasrum; Kurt Allen Krobert; Soheil Naderi; Lise Román Moltzau
• Keywords: NPR2; NPR1; Expression (computer science)

<p class="first" dir="auto" id="d3301962e120">Natriuretic peptides (NPs) are cardiac hormones that play a significant role in functioning of the heart and promising for treatment failure. NPs increase cGMP levels by activating two NP receptors, NPR-A (ANP BNP) NPR-B (CNP). However, influence these receptors on contractility various intracellular targets is not same. Our aim to study if activity NPRs differentially regulated interacting proteins, cytohesins. id="d3301962e122">By Western blotting qPCR we found cytohesins 1-4 were expressed rat mouse ventricular cardiomyocytes, several cell lines different compartments heart. Besides, observed expression cytohesin-3 -4 was significantly altered failure model. Using co-immunoprecipitation microscale thermophoresis, demonstrated cytohesin-2 interacted with both NPR-B. peptide array assay, identified sequences from involved interaction. Furthermore, cytohesin inhibitor SecinH3 produced cardiomyocytes lines. Finally, reduced CNP-induced negative inotropic response but enhanced its lusitropic effect isolated left muscle strips. id="d3301962e124">These results provide novel information about their connection natriuretic system, supporting our hypothesis may be regulation activity.