Fecal Microbiota Transplantation Attenuates Frailty via Gut-Muscle Axis in Old Mice

• DOI: 10.14336/ad.2024.0321
• Publication Date: 2024-04-22T17:30:51Z
• Authors: Mengpei Zhu; Yumei Huang; Ziwen Wang; Ze Jin; Jiali Cao; Qiangqiang Zhong; Zhifan Xiong
• Keywords: Fecal bacteriotherapy; Gut–brain axis

Targeting adverse pathogenic gut microbiota regulation through fecal transplantation (FMT) may restore health and has been validated in some aging-related diseases. However, the mechanisms of microbiota's role frailty whether modulation can treat age-related remain largely unknown. To assess effects FMT on frailty, we used bidirectional young old mice. We demonstrated that bacteria transplanted from mice into reduced body weight grip strength (p=0.002), led to elevated inflammatory factors mice, but had no significant effect intestinal barrier function. Notably, treatment older not only improved (grip strength: p=0.036, low physical activity: p=0.020, running speed: p=0.048, time: p=0.058, score: p=0.027) muscle mass, also ecological imbalances, function, systemic inflammation (serum TNF-α: p=0.002, IL-6: p<0.001). KEGG enrichment analysis metabolites showed ameliorate sphingolipid metabolism pathway. In addition, aged given a increase abundance SCFA-producing increased levels short-chain fatty acids (butyric acid: p=0.084, propionic p=0.028). Subsequent further verification found ameliorating be achieved SCFAs metabolism. Another mechanism study reduces lipopolysaccharide (p<0.001), thereby inhibiting TLR4/NF-κB signaling pathway its downstream pro-inflammatory products. Therefore, regulating by altering microbial composition targeting gut-muscle axis with LPS/TLR4 pathways potential strategies adults.