The Histone Demethylase PHF2 Promotes Fat Cell Differentiation as an Epigenetic Activator of Both C/EBPα and C/EBPδ

• DOI: 10.14348/molcells.2014.0180
• Publication Date: 2014-09-29T10:36:09Z
• Authors: Kyoung-Hwa Lee; Uk-Il Ju; Jung-Yup Song; Yang-Sook Chun
• Keywords: Demethylase; Chromatin immunoprecipitation; Histone Methylation

Histone modifications on major transcription factor target genes are one of the regulatory mechanisms controlling adipogenesis. Plant homeodomain finger 2 (PHF2) is a Jumonji domain-containing protein and known to demethylate histone H3K9, repressive gene marker. To better understand function PHF2 in adipocyte differentiation, we constructed stable knock-down cells by using mouse pre-adipocyte cell line 3T3-L1. When induced with adipogenic media, showed reduced lipid accumulation compared control cells. Differential expression cDNA microarray revealed significant reduction metabolic pathway after differentiation. The factors adipokines was confirmed reverse transcription- quantitative polymerase chain reaction Western blotting. We further performed co-immunoprecipitation analysis four factors, found that CCATT/enhancer binding (C/EBP)α C/EBPδ physically interact PHF2. In addition, promoters chromatin immunoprecipitation experiment. Finally, H3K9 methylation markers PHF2-binding sequences were increased Together, these results demonstrate demethylase controls during