Single-cell analyses EMP1 as a marker of the ratio of M1/M2 macrophages is associated with EMT, immune infiltration, and prognosis in bladder cancer

• DOI: 10.14440/bladder.2023.852
• Publication Date: 2023-12-18T02:06:45Z
• Authors: Jinqiao Li; Jianyu Liu; Honglei Wang; Jinpeng Ma; Yueze Wang; Wanhai Xu
• Keywords: Infiltration (HVAC)

Background: Bladder cancer is among the most lethal urinary system cancers across globe. Macrophage 1 and 2 play an essential role in pathogenesis of tumors. Nevertheless, prior studies failed to investigate implication two cells, working combination, development, growth, progression metastasis bladder cancer.   Methods: We computed M1/M2 ratio samples retrieved from The Cancer Genome Atlas (TCGA) by using Cibersortx algorithm calculated 32 patients our series employing flow cytometry. SurvivalRandomForest was utilized reduce dimension list M1/M2-related genes, with aim obtain survival-predictive gene (EMP1) encoding epithelial membrane protein (EMP1). EMP1 biologically characterized Gene Set Enrichment Analysis (GSEA), Variation (GSVA), Ontology (GO). single-cell transcriptome (sc-RNA) analysis then applied further look into function of EMP1. Finally, Cellchat employed examine interaction between macrophages epithelium cells. Results: The results showed that higher found be associated a more favorable prognosis cancer. EMP1 was identified key indicative higher EMP1 expression poor prognosis. Further analyses that EMP1 might promote tumor invasion via epithelial-mesenchymal transition (EMT) focal adhesion (FA). Moreover, expression level of EMP1 could serve as indicator immunotherapy efficacy. scRNA-seq data indicated that EMP1 in cells strongly proliferation. exhibited through fibronectin 1-syndecan (FN1-SDC1) pathway. Conclusion: Our study identified EMP1, gene, which may act prognostic for proliferation, metastasis, response immunotherapy. EMP1 might involved regulation on ratio.