Using a variant of the optomotor response as a visual defect detection assay in zebrafish

• DOI: 10.14440/jbm.2021.341
• Publication Date: 2021-03-18T04:48:49Z
• Authors: Matthew K. LeFauve; Cassie J. Rowe; Mikayla Crowley-P...; Jenna L. Wiegand; Arthur G. Shapiro
• Keywords: Stimulus (psychology); Danio; Photic Stimulation

We describe a visual stimulus that can be used with both larval and adult zebrafish (Danio rerio). This protocol is modification of standard behavior analysis, the optomotor response (OMR). The OMR often to determine spatial or detect directional visuomotor deficiencies. An generated using high contrast grated pattern, typically vertical bars. sensitivity measured by detection change in grating bar width reported cycles per degree (CPD). test has been extensively larvae adults identify visual- and/or motor-based mutations. Historically, when tested adults, pattern was presented from perspective, rotating cylinder around holding tank, allowing seen solely sides front organism. In contrast, OMRs are elicited projected below fish. difference methodology means two different experimental set-ups required: one for larvae. Our modifies stimulation format so single stimulus, suitable use larvae, being underneath Analysis responses this method does not require costly behavioral tracking software and, paradigm, allows observer rapidly adults.