lncRNA PCAT1 might coordinate ZNF217 to promote CRC adhesion and invasion through regulating MTA2/MTA3/Snai1/E-cadherin signaling

• DOI: 10.14715/cmb/2021.67.4.1
• Publication Date: 2022-01-10T13:52:57Z
• Authors: Shulong Zhang; Kaihua Zhu; Qi Han; Quan Wang; Bin Yang
• Keywords: Male; 0301 basic medicine; Cadherins; Histone Deacetylases; Neoplasm Proteins; 3. Good health; Gene Expression Regulation, Neoplastic; Repressor Proteins; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Trans-Activators; Humans; RNA, Long Noncoding; Snail Family Transcription Factors; Colorectal Neoplasms; Cell Proliferation

LncRNA prostate cancer-associated transcript 1 (PCAT1) is a well-known oncogene, but the mechanisms of exosomes PCAT1 in colorectal cancer (CRC) remain largely unknown. Thus, lncRNA were investigated. The expressions tissues from stage 0-I and II-III CRC patients, intestinal epithelial cell line FHC two lines, HT29 HCT8 measured by real-time quantitative PCR. effects on adhesion invasion lines investigated cell-matrix transwell assays. In addition, target (ZNF217) was validated using an RNA immune precipitation assay. Finally, protein levels MTA2, MTA3, SNAI1, E-cadherin normal participants, as well with stable ZNF217 knockdown western blotting. plasma exosomal found to be significantly increased lines. inhibited cells. RIP assay results showed could ZNF217, downregulation decrease cells Moreover, decreased SNAI1 expressions, both Exosomal can promote cells, overexpression may lead upregulation that regulates EMT-related MTA2/MTA3/Snai1/E-cadherin signaling.