MicroRNA-208b progressively declines after spinal cord injury in humans and is inversely related to myostatin expression

• DOI: 10.14814/phy2.12622
• Publication Date: 2015-11-25T01:25:32Z
• Authors: Hanneke Boon; Rasmus J. O. Sjögren; Julie Massart; Brendan Egan; Emil Kostovski; Per O. Iversen; Nils Hjeltnes; Alexander V. Chib...; Ulrika Widegren; Juleen R. Zierath
• Keywords: Myostatin; Muscle Atrophy

The effects of long-term physical inactivity on the expression microRNAs involved in regulation skeletal muscle mass humans are largely unknown. MicroRNAs short, noncoding RNAs that fine-tune target through mRNA degradation or by inhibiting protein translation. Intronic to slow, type I, fiber genes MYH7 and MYH7b, microRNA-208b microRNA-499-5p thought important for growth, such as myostatin. Spinal cord injured characterized both atrophy transformation toward fast-twitch, II fibers. We determined microRNA-208b, microRNA-499-5p, myostatin human after complete cervical spinal injury. also whether these altered rodent muscle. A progressive decline occurred during first year injury with long-standing Expression was inversely correlated Overexpression intact mouse decreased expression, whereas without effect. In conclusion, we provide evidence an inverse relationship between its previously validated severe atrophy. Moreover, direct overexpression decreases gene Our results implicate modulates this may play a role following