Regulation of PPARGC1A gene expression in trained and untrained human skeletal muscle

• DOI: 10.14814/phy2.13543
• Publication Date: 2017-12-13T01:10:11Z
• Authors: Daniil V. Popov; Evgeny A. Lysenko; Pavel A. Makhnovskii; Nadia S. Kurochkina; Olga L. Vinogradova
• Keywords: PPARGC1A

Promoter-specific expression of the PPARGC1A gene in untrained and trained human skeletal muscle was investigated. Ten males performed a one-legged knee extension exercise (for 60 min) with same relative intensity both before after 8 weeks cycling training. Samples from m. vastus lateralis each leg were taken exercise. Postexercise via canonical promoter increased by ~100% (P < 0.05) exercised nonexercised muscles, but did not change either training program. In muscle, alternative two orders magnitude 0.01). We found increases postexercise content dephosphorylated (activated) CRTC2, coactivator CREB1, CREB1-related genes 0.01–0.05); this may partially explain promoter. addition, comparison regulatory regions promoters revealed unique conserved motifs that associated transcriptional repressors SNAI1 HIC1. conclusion, exercise-induced be regulated systemic factors, while shows constitutive at rest Exercise-induced relates to intramuscular factors associates activation CRTC2-CREB1. Apparently, is other transcription particularly repressors.