Regular exercise and branched‐chain amino acids prevent ischemic acute kidney injury‐related muscle wasting in mice

• DOI: 10.14814/phy2.14557
• Publication Date: 2020-08-29T08:49:47Z
• Authors: Soichiro Nagata; Akihiko Kato; Shinsuke Isobe; Tomoyuki Fujikura; Naro Ohashi; Hiroaki Miyajima; Hideo Yasuda
• Keywords: Male; 0301 basic medicine; Physiology; Muscle Proteins; Mice; 03 medical and health sciences; Physical Conditioning, Animal; QP1-981; Animals; Muscle, Skeletal; Original Research; branched‐chain amino acid; SKP Cullin F-Box Protein Ligases; exercise; Wasting Syndrome; muscle wasting; Acute Kidney Injury; Mitochondria, Muscle; 3. Good health; Mice, Inbred C57BL; PPAR gamma; acute kidney injury; Proteolysis; Proto-Oncogene Proteins c-akt; Amino Acids, Branched-Chain

Acute kidney injury (AKI) causes glucose and protein metabolism abnormalities that result in muscle wasting, thereby affecting the long-term prognosis of critical illness survivors. Here, we examined whether early intervention with treadmill exercise branched-chain amino acids (BCAA) can prevent AKI-related wasting reduced physical performance mice. Unilateral 15 min ischemia-reperfusion was induced contralateral nephrectomized mice, histological physiological changes were assessed compared those pair-fed control since AKI severe anorexia. Mice exercised for 30 each day received oral BCAA 7 days after insult. By 7, ischemic significantly decreased wet weight, myofiber cross-sectional area, central mitochondrial volume density anterior tibialis muscle, maximal time. Regular prevented low by suppressing myostatin atrogin-1 mRNA upregulation, restoring phosphorylated Akt PGC-1α expression muscle. Ischemic induces accelerating degradation reducing synthesis; however, found regular without worsening damage, suggesting rehabilitation nutritional support could wasting.