Spontaneous restoration of functional β‐cell mass in obese SM/J mice

• DOI: 10.14814/phy2.14573
• Publication Date: 2020-10-28T21:02:06Z
• Authors: Mario A. Miranda; Caryn Carson; Celine L. St. Pierre; Juan F. Macias‐Ve...; Jing W. Hughes; Marcus Kunzmann; Heather Schmidt; Jessica P. Wayhart; Heather A. Lawson
• Keywords: Male; 0301 basic medicine; insulin; obesity; 571; Physiology; mouse model; Diet, High-Fat; Mice; 03 medical and health sciences; Insulin-Secreting Cells; β‐cell mass; Insulin Secretion; QP1-981; Animals; Obesity; Cells, Cultured; Original Research; Cell Proliferation; 2. Zero hunger; 0303 health sciences; diabetes; Glucagon-Secreting Cells; Female; hyperglycemia

Maintenance of functional β-cell mass is critical to preventing diabetes, but the physiological mechanisms that cause populations thrive or fail in context obesity are unknown. High fat-fed SM/J mice spontaneously transition from hyperglycemic-obese normoglycemic-obese with age, providing a unique opportunity study adaptation. Here, we characterize insulin homeostasis, islet morphology, and function during SM/J's diabetic remission. As they resolve hyperglycemia, obese dramatically increase circulating pancreatic levels while improving sensitivity. Immunostaining sections reveals selectively not α-cell mass. Obese do show elevated mitotic index, rather index. Functional assessment isolated islets glucose-stimulated secretion, decrease basal content. These results establish expansion improved underlie resolution indicating valuable tool for exploring how can be recovered obesity.