Visualization of cardiac uptake of bone marrow mesenchymal stem cell‐derived extracellular vesicles after intramyocardial or intravenous injection in murine myocardial infarction

• DOI: 10.14814/phy2.15568
• Publication Date: 2023-03-27T02:50:21Z
• Authors: Cynthia M. Xu; Sharif A. Sabe; Rayane Brinck‐Tei...; Mohamed Sabra; Frank W. Sellke; M. Ruhul Abid
• Keywords: Biodistribution; Miniature swine

Abstract In animal models, human bone marrow mesenchymal stem cell‐derived extracellular vesicles (MSC‐EV) have been found to beneficial effects in cardiovascular disease, but only when administered via intramyocardial injection. The biodistribution of either intravenous or injection MSC‐EV the presence myocardial injury is uncharacterized at this time. We hypothesized that will ensure delivery ischemic myocardium, while not. Human cells were cultured and isolated characterized. MSC‐EVs then labeled with DiD lipid dye. FVB mice normal cardiac function underwent left coronary artery ligation followed by peri‐infarct tail vein 3*10 6 2*10 9 particles DiD‐labeled a DiD‐saline control. heart, lungs, liver, spleen kidneys harvested 2 h post‐injection submitted for fluorescent molecular tomography imaging. Myocardial uptake was visualized after ( p = 0.01) compared control, there no differences fluorescence 0.5). There significantly detectable other organs After MSC‐EV, liver 0.02) 0.04) appeared diffuse controls. Even injury, not administration resulted levels myocardium. This study confirms role maximal effective MSC‐EV. Our ongoing studies aimed developing bioengineered targeted heart may render clinically applicable disease.