Epstein-Barr virus reactivation in peripheral B lymphocytes induces IgM-type thyrotropin receptor autoantibody production in patients with Graves’ disease

• DOI: 10.1507/endocrj.ej22-0609
• Publication Date: 2023-03-09T22:13:36Z
• Authors: Keiko Nagata; Kazuhiko Hayashi; Keisuke Kumata; Yukio Satoh; Mitsuhiko Osaki; Yuji Nakayama; Satoshi Kuwamoto; Yoshinori Ichihara; Tsuyoshi Okura; Kazuhiko Matsuzawa; Junichiro Miake; Shuji Fukata; Takeshi Imamura
• Keywords: Trab

Epstein-Barr virus (EBV) is a human herpes that latently infects B lymphocytes. When EBV reactivated, host cells differentiate into plasma and produce IgM-dominant antibodies as well many progeny virions. The aims of the present study were to confirm IgM dominance thyrotropin-receptor (TRAbs) produced by reactivation investigate roles TRAb-IgM in Graves' disease. Peripheral blood mononuclear (PBMCs) containing TRAb-producing stimulated for reactivation, TRAb-IgG measured ELISA. purified TSH-binding inhibitory activities assessed using radio-receptor assay. Porcine thyroid follicular epithelial cultured with and/or complements measure intracellular levels cAMP amount LDH released. TRAb-IgM/TRAb-IgG (the MG ratio) was examined sequential serum samples disease compared among groups function. results obtained showed TRAb production induced reactivation. did not inhibit TSH binding receptors transduce hormone-producing signals. However, it destroyed complements. ratio significantly higher hyperthyroidism or hypothyroidism than those normal function healthy controls. A close relationship observed between development exacerbation provide novel insights prophylaxis therapeutics