Treatment of adhesions after Achilles tendon injury using focused ultrasound with targeted bFGF plasmid-loaded cationic microbubbles

• DOI: 10.1515/chem-2020-0110
• Publication Date: 2020-06-17T13:01:18Z
• Authors: Yuzhou Shen; Jiancheng Ma; Junsheng Jiang; Zhilin Chen; Wenzhu Yan; Yue Wang; Feng Wang; Li Liu
• Keywords: Sonoporation

Abstract Nonviral targeting technology has become promising as a form of gene therapy for diseases and injuries, such Achilles tendon injuries. In this study, we used avidin–biotin bridge positive–negative charge attractions to load the intercellular adhesion molecule-1 (ICAM-1) antibody basic fibroblast growth factor (bFGF) plasmid onto surface microbubbles. The saturated loading capacity 1.1 × 10 8 microbubble was 6.55 ± 0.53 µg. We established ICAM-1 antigen microenvironment using tumor necrosis factor-alpha-stimulated human umbilical vein endothelial cells found ability prepared microbubbles in vitro . vivo , also that injected targeted bFGF expressed better when compared with control group. Furthermore, evaluated adhesions after injuries rabbits hematoxylin eosin immunohistochemical (IHC) staining methods. collagen fibers were properly arranged tendon, there greater cellularity inside sheath clearer boundary between internal external than IHC showed expression outside sheath. conclusion, can be useful carrier genes provide prevention injury.