Differential effects of choline on TLR2/4 mediated signaling through possible regulation of Toll-interacting protein in hepatocellular carcinoma cell lines

• DOI: 10.1515/tjb-2023-0282
• Publication Date: 2024-05-24T11:50:31Z
• Authors: Elif Baris; Ayse Banu Demir
• Keywords: 0301 basic medicine; 0303 health sciences; choline; inflammation; 610; toll-like receptor; Naturwissenschaften; hepatocellular carcinoma; QD415-436; toll-interacting protein; Biochemistry

Abstract Objectives Toll-like receptor (TLR) mediated inflammatory status plays an important role in development and progression of hepatocellular carcinoma (HCC). Toll-interacting protein (TOLLIP) has inhibitory effect on TLR-mediated signalling expression profile TOLLIP varies between malignancies including HCC. Cholinergic anti-inflammatory pathway (CAP) is endogenous mechanism that controls via α7nicotinic acetylcholine receptors (α7nAChR). This study aims to investigate the CAP-acting agent choline its related response HCC cells with distinct differentiation stages. Methods The patterns α7nAChR , TLR2 / 4 IL6, NFkB genes were evaluated by RT-PCR ELISA presence choline, along real-time cell proliferation migration HEP3B SNU449 lines. interaction assessed using in-silico analyses. Results Choline downregulated Hep3B cells. However, expressions NF-κB IL-6 TLR4 showed a decreased pattern well differentiated cells, while increased poorly Conclusions might exert differential effects TLR2/4-dependent based stages suggesting potential therapeutic earlier which be result partial modulation TOLLIP.