Differential effects of choline on TLR2/4 mediated signaling through possible regulation of Toll-interacting protein in hepatocellular carcinoma cell lines
0301 basic medicine
0303 health sciences
choline
inflammation
610
toll-like receptor
Naturwissenschaften
hepatocellular carcinoma
QD415-436
toll-interacting protein
Biochemistry
DOI:
10.1515/tjb-2023-0282
Publication Date:
2024-05-24T11:50:31Z
AUTHORS (2)
ABSTRACT
Abstract Objectives Toll-like receptor (TLR) mediated inflammatory status plays an important role in development and progression of hepatocellular carcinoma (HCC). Toll-interacting protein (TOLLIP) has inhibitory effect on TLR-mediated signalling expression profile TOLLIP varies between malignancies including HCC. Cholinergic anti-inflammatory pathway (CAP) is endogenous mechanism that controls via α7nicotinic acetylcholine receptors (α7nAChR). This study aims to investigate the CAP-acting agent choline its related response HCC cells with distinct differentiation stages. Methods The patterns α7nAChR , TLR2 / 4 IL6, NFkB genes were evaluated by RT-PCR ELISA presence choline, along real-time cell proliferation migration HEP3B SNU449 lines. interaction assessed using in-silico analyses. Results Choline downregulated Hep3B cells. However, expressions NF-κB IL-6 TLR4 showed a decreased pattern well differentiated cells, while increased poorly Conclusions might exert differential effects TLR2/4-dependent based stages suggesting potential therapeutic earlier which be result partial modulation TOLLIP.
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