Human Cortical Neurons Originate from Radial Glia and Neuron-Restricted Progenitors

• DOI: 10.1523/jneurosci.0111-07.2007
• Publication Date: 2007-04-11T16:58:06Z
• Authors: Zhicheng Mo; Anna R. Moore; Radmila Filipovic; Yasuhiro Ogawa; Ikenaka Kazuhiro; Srdjan D. Antic; Nada Zecevic
• Keywords: Progenitor; PAX6; Ganglionic eminence

Understanding the molecular and physiological determinants of cortical neuronal progenitor cells is essential for understanding development human brain in health disease. We used surface marker fucose N -acetyl lactosamine (LeX) (also known as CD15) to isolate from ventricular/subventricular zone fetal at second trimester gestation study their progeny vitro . LeX + had typical bipolar morphology, radial orientation, antigen profiles, characterizing them a subtype glia (RG) cells. Four complementary experimental techniques (clonal analysis, immunofluorescence, transfection experiments, patch-clamp recordings) indicated that this RG generates mainly astrocytes but also small number neurons. The neurogenic capabilities RGs were both region stage dependent. Present results provide first direct evidence cerebral cortex serve progenitors. Simultaneously, another was identified proliferating labeled with (β-III-tubulin doublecortin) not markers [GFAP, vimentin, BLBP (brain lipid-binding protein)]. Proliferative antigenic characteristics these suggested neuron-restricted status. In summary, our suggests diverse populations cells, including multipotent progenitors, contribute differentially neurogenesis cortex.