Synaptic Glutamate Release Is Modulated by the Na+-Driven Cl−/HCO3−Exchanger Slc4a8

• DOI: 10.1523/jneurosci.0269-11.2011
• Publication Date: 2011-05-18T16:53:15Z
• Authors: Anne Sinning; Lutz Liebmann; Alexandra Kougiou...; Martin Westermann; Claus Bruehl; Christian A. Hübner
• Keywords: Intracellular pH

On the one hand, neuronal activity can cause changes in pH; on other pH modulate activity. Consequently, of brain is regulated at various levels. Here we show that steady-state and acid extrusion were diminished cultured hippocampal neurons mice with a targeted disruption Na + -driven Cl − /HCO 3 exchanger Slc4a8. Because Slc4a8 was found to predominantly localize presynaptic nerve endings, hypothesize key regulator pH. Supporting this hypothesis, spontaneous glutamate release CA1 pyramidal layer reduced but could be rescued by increasing intracellular The excitability vitro correlated an increased seizure threshold vivo . Together altered kinetics stimulated synaptic vesicle release, these data suggest modulates pH-dependent manner.