Neurotrophic activity in the adult rat hippocampal formation: regional distribution and increase after septal lesion

• DOI: 10.1523/jneurosci.05-10-02809.1985
• Publication Date: 2018-03-30T18:30:58Z
• Authors: F Collins; KA Crutcher
• Keywords: Sprouting; Neurite

Conditioned medium prepared from slices of the rat hippocampal formation contains an agent that shares following properties with nerve growth factor (NGF): it promotes neurite embryonic sympathetic ganglia in vitro; and is inhibited by affinity-purified antibody against mouse submaxillary gland 2.5 S NGF. The NGF-like growth-promoting activity regionally distributed within formation: consistently higher dentate gyrus-CA3 region than CA1 region. Furthermore, level significantly increased 1 week after a medial septal lesion, and, dentate-CA3 region, maintained for at least 4 weeks lesion. Control lesions fail to interrupt septohippocampal innervation, but cause equally extensive damage nearby regions central nervous system, do not levels formation. These results provide substantial evidence linking conditioned sprouting axons into lesion vivo.