Selective and nonselective stimulation of central cholinergic and dopaminergic development in vitro by nerve growth factor, basic fibroblast growth factor, epidermal growth factor, insulin and the insulin-like growth factors I and II

• DOI: 10.1523/jneurosci.10-02-00558.1990
• Publication Date: 2018-04-02T21:55:42Z
• Authors: B Knusel; PP Michel; JS Schwaber; F Hefti
• Keywords: Ciliary neurotrophic factor

To study the selectivity of neurotrophic actions in brain, we analyzed several known growth factors on septal cholinergic, pontine and mesencephalic dopaminergic neurons culture. Similar to nerve factor (NGF), basic fibroblast (bFGF) stimulated choline acetyltransferase activity cultures. In contrast NGF, bFGF also enhanced dopamine uptake cultures cell proliferation all 3 culture types. Insulin insulin-like I II transmitter-specific differentiation Epidermal (EGF) produced a small increase by cells cultures, was most effective when given at early times, NGF later times. The stimulatory insulin did not require presence glial were mediated NGF. stimulation EGF dependent proliferation. results suggest different degrees molecules. and, very similarly, seem influence cholinergic directly rather selectively, whereas appear be more general.