Localization of dopamine D2 receptor mRNA and D1 and D2 receptor binding in the rat brain and pituitary: an in situ hybridization- receptor autoradiographic analysis

• DOI: 10.1523/jneurosci.10-08-02587.1990
• Publication Date: 2018-04-04T17:35:38Z
• Authors: A Mansour; JH Meador-Woodruff; JR Bunzow; O Civelli; H Akil; SJ Watson
• Keywords: Zona incerta; Raclopride

Several lines of evidence suggest the existence multiple dopamine receptor subtypes, referred to as D1 and D2. The present study examines distribution these binding sites in rat brain pituitary relation D2 mRNA using a combination vitro autoradiographic situ hybridization techniques. 3H-Raclopride 3H-SCH23390 (in presence 1 microM ketanserin) were used label sites, respectively, while 495 bp cRNA probe synthesized from Sac I-Bgl II fragment cDNA was visualize mRNA. Analysis adjacent tissue sections which autoradiography had been performed revealed several regions where site corresponding appear be similarly distributed, including caudate-putamen, nucleus accumbens, olfactory tubercle, globus pallidus, substantia nigra, ventral tegmental area. In gland, codistributed with very dense levels intermediate lobe individually labeled cells anterior lobe. Brain demonstrating lack correspondence between include bulb, neocortex, paleocortex, hippocampus, zona incerta. hypotheses are discussed explain certain regions; localization on both fibers cell bodies transport. These studies provide better understanding anatomical serve framework for future regulatory mapping studies. By focusing specific regions, such nigrostriatal system, they insights into synthesis, transport, insertion membranes.