Cellular and molecular characterization of a brain-enriched protein tyrosine phosphatase

• DOI: 10.1523/jneurosci.15-02-01532.1995
• Publication Date: 2018-04-02T18:00:44Z
• Authors: LM Boulanger; PJ Lombroso; A Raghunathan; MJ During; P Wahle; JR Naegele
• Keywords: Putamen

Regional variations in the expression of a striatal enriched protein tyrosine phosphatase called STEP were studied adult rat brain by combination immunocytochemistry, lesion studies, Western blotting, and situ hybridization. Monoclonal antibodies generated against identified multiple polypeptides M(r) 46, 37, 33 doublet 64-66 kDa on blots. Although three immunoreactive bands with lower molecular weights cytosolic fractions, was membrane fractions. All forms abundant caudate-putamen present amounts or undetectable other regions. In substantia nigra, not detected but present. Immunocytochemical experiments demonstrated that isoforms nigra are presynaptic axons originating from projection neurons caudate putamen, which innervate this structure. Additional hybridization studies showed mRNA patterns correlate immunocytochemical staining. These findings indicate there polypeptide basal ganglia related structures differ terms their intracellular locations functional roles.