TRPM1 Forms Complexes with NyctalopinIn Vivoand Accumulates in Postsynaptic Compartment of ON-Bipolar Neurons in mGluR6-Dependent Manner

• DOI: 10.1523/jneurosci.1682-11.2011
• Publication Date: 2011-08-10T19:52:10Z
• Authors: Yan Cao; Ekaterina Posokhova; Kirill A. Martemy...
• Keywords: Compartment (ship)

Synaptic transmission between light-sensory photoreceptor cells and downstream ON-bipolar neurons plays an important role for vertebrate vision. This process is mediated by the G-protein-coupled receptor pathway involving glutamate mGluR6 effector channel TRPM1. The signal occurs on a rapid timescale; however, molecular organization that ensures timely signaling in this cascade unknown. Genetic studies human patients animal models reveal cell depends synaptic protein nyctalopin. We have conducted proteomic search proteins associated with nyctalopin mouse retina identified TRPM1 as binding partner. further demonstrate additionally interacts receptor. Disruption of prevented targeting to postsynaptic compartment neurons. These results unique macromolecular cascade, where principal components are scaffolded nyctalopin, creating essential correct localization ensemble ultimately intact at first visual synapse.