Neuronal Regulation of Glutamate Transporter Subtype Expression in Astrocytes

• DOI: 10.1523/jneurosci.17-03-00932.1997
• Publication Date: 2018-04-03T15:00:43Z
• Authors: Raymond A. Swanson; Jialing Liu; Johann W. Miller; Jeffrey D. Rothstein; Kevin Farrell; Becky A. Stein; Maria C. Longuemare
• Keywords: Glutamate aspartate transporter; Neuroglia

GLT-1, GLAST, and EAAC1 are high-affinity, Na + -dependent glutamate transporters identified in rat forebrain. The expression of these transporter subtypes was characterized three preparations: undifferentiated cortical astrocyte cultures, astrocytes cocultured with neurons, cultures differentiated dibutyryl cyclic AMP (dBcAMP). monocultures expressed only the GLAST subtype. Astrocytes neurons developed a stellate morphology both GLT-1; transporter, rare microglia GLT-1. Treatment dBcAMP induced GLT-1 increased GLAST. These effects on were qualitatively similar to those resulting from coculture but immunocytochemistry showed pattern be more complex preparations. Compared expressing dBcAMP-treated an increase uptake V max , no change K m sensitivity inhibition by dihydrokainate. Pyrrolidine-2,4-dicarboxylic acid threo -β-hydroxyaspartic caused relatively less transport suggesting weaker effect at than studies show that is influenced can partially mimic this influence. Manipulation may permit identification factors regulating function their native cell type.