p53 Expression Induces Apoptosis in Hippocampal Pyramidal Neuron Cultures

• DOI: 10.1523/jneurosci.17-04-01397.1997
• Publication Date: 2018-04-03T15:01:02Z
• Authors: Joaquı́n Jordán; Marı́a F. Galindo; Jochen H. M. Prehn; Ralph R. Weichsel...; Michael Beckett; Ghanashyam D. Ghadge; Raymond P. Roos; Jeffrey M. Leiden; Richard J. Miller
• Keywords: Neurons; 0301 basic medicine; Pyramidal Cells; Cell Cycle; Apoptosis; Hippocampus; Rats; 03 medical and health sciences; Transforming Growth Factor beta; Animals; Genes, Tumor Suppressor; Tumor Suppressor Protein p53; Cells, Cultured

The tumor suppressor gene p53 has been implicated in the induction of apoptosis dividing cells. We now show that overexpression using an adenoviral vector cultured rat hippocampal pyramidal neurons causes widespread neuronal death with features typical apoptosis. did not induce p21, bax, or mdm2 neurons. X-irradiation induced immunoreactivity and cell associated Overexpression a constitutively active nonphosphorylatable form retinoblastoma product blocked x-irradiation-induced death. However, cyclin-dependent kinase inhibitor p21 not. Treatment transforming growth factor-β1 protected them from x-irradiation. These results are consistent role for nerve is distinct its actions relating to cycle arrest.