BDNF Regulates the Translation of a Select Group of mRNAs by a Mammalian Target of Rapamycin-Phosphatidylinositol 3-Kinase-Dependent Pathway during Neuronal Development

• DOI: 10.1523/jneurosci.1739-04.2004
• Publication Date: 2004-08-18T18:51:44Z
• Authors: Gerhard M. Schratt; Elizabeth A. Nigh; Wen G. Chen; Linda Hu; Michael E. Greenberg
• Keywords: Neurons; 0301 basic medicine; 0303 health sciences; Brain-Derived Neurotrophic Factor; Gene Expression Profiling; TOR Serine-Threonine Kinases; Brain; Dendrites; Rats; Phosphatidylinositol 3-Kinases; 03 medical and health sciences; Gene Expression Regulation; Homer Scaffolding Proteins; Polyribosomes; Protein Biosynthesis; Synapses; Animals; Rats, Long-Evans; RNA, Messenger; Carrier Proteins; Protein Kinases; Signal Transduction

Local regulation of mRNA translation plays an important role in axon guidance, synaptic development, and neuronal plasticity. Little is known, however, regarding the mechanisms that control neurons, only a few mRNAs have been identified are locally translated within dendrites. Using Affymetrix gene arrays to identify newly associated with polysomes after exposure BDNF, we subsets for which enhanced neurons at different developmental stages. In mature many these encode proteins known function synapses, including CamKIIalpha, NMDA receptor subunits, postsynaptic density (PSD) scaffolding protein Homer2. BDNF regulates Homer2 synaptodendritic compartment by activating translational initiation via mammalian target rapamycin-phosphatidylinositol 3-kinase-dependent pathway. These findings suggest likely inducing local synthesis numerous proteins. The cytoskeleton-associated particular might implications growth cone dynamics dendritic spine development.