Cytoskeleton Regulation of Glycine Receptor Number at Synapses and Diffusion in the Plasma Membrane

• DOI: 10.1523/jneurosci.1758-06.2006
• Publication Date: 2006-08-16T19:42:55Z
• Authors: Cécile Charrier; Marie-Virginie Eh...; Maxime Dahan; Sabine Lévi; Antoine Triller
• Keywords: Gephyrin; Neurotransmitter receptor

Lateral diffusion of neurotransmitter receptors in and out synapses has been postulated as a core mechanism for rapid changes receptor number at during plastic processes. In this study, we have used single particle tracking to investigate how glycine (GlyR) lateral properties might account after disruption the cytoskeleton dissociated spinal cord neurons. We found that pharmacological F-actin microtubules decreased amount GlyR gephyrin, backbone inhibitory postsynaptic scaffold, synapses. microtubule increased exchanges between synaptic extrasynaptic membranes dwell time was predominantly controlled by membrane actin Both coefficients confinement were affected disruption. Our results indicate compartments depend on both differentiation membrane. Consequently, may be rapidly modulated through regulation plasma stabilization